| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062758 |
| Chromosome: | chromosome 3 |
| Location: | 1857956 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154700 | (1 of 3) PF01937 - Protein of unknown function DUF89 (DUF89) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGCGGGGCTGGGGAAGCGGAGGGGCGG |
| Internal bar code: | ACAACAAAATGTATCTGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 56 |
| LEAP-Seq percent confirming: | 98.7179 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGCAGATCTCCTGGTTC |
| Suggested primer 2: | ATGTAACGAGCGTGGGGTAG |