| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062787 |
| Chromosome: | chromosome 10 |
| Location: | 4366786 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451300 | EXN13 | (1 of 3) PTHR13620//PTHR13620:SF0 - 3-5 EXONUCLEASE // EXONUCLEASE 3'-5' DOMAIN-CONTAINING PROTEIN 2; 3'-5' exonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTTAGGAAGGCGCGCGAAGTGTGGACT |
| Internal bar code: | TCACGTCGGACGCCGTGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 792 |
| LEAP-Seq percent confirming: | 99.496 |
| LEAP-Seq n confirming: | 4935 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCGTCGTATAATCTGCG |
| Suggested primer 2: | ATGACGAAACCGAAACCAAG |