| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062807 |
| Chromosome: | chromosome 13 |
| Location: | 414559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564650 | MRS5 | Magnesium and cobalt transport protein; (1 of 3) 3.6.3.2 - Magnesium-importing ATPase / Mg(2+)-importing ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGGCGCCGCGGCCGCACCTACCGCAA |
| Internal bar code: | GGCCGGACGCCCAAGCAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTACACGGCAACAGGTC |
| Suggested primer 2: | TTATCCTGCGGCAGAGTACC |