| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062825 |
| Chromosome: | chromosome_1 |
| Location: | 5705136 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g040650 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGATATGCAGTGAAGGGGAAATGCCGGGG |
| Internal bar code: | TCCGTCGGGGGGAAACGTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.2751 |
| LEAP-Seq n confirming: | 2739 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCTACATCTGCTGACGA |
| Suggested primer 2: | CAGCAGTGGCATAATGGATG |