| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.062839 |
| Chromosome: | chromosome 15 |
| Location: | 777066 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640101 | (1 of 3) IPR000104//IPR006553 - Antifreeze protein, type I // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGGGCAACCGCCGCCATGCCTGTGCGG |
| Internal bar code: | AAGACAGCTCGGAGTAGAGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 97.8158 |
| LEAP-Seq n confirming: | 7479 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTCGGTGGGTAGGTGAG |
| Suggested primer 2: | TTCAACCGACACCACCACTA |