| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062841 |
| Chromosome: | chromosome 3 |
| Location: | 1135478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149150 | (1 of 1) IPR000104//IPR013022 - Antifreeze protein, type I // Xylose isomerase-like, TIM barrel domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACTGCTGCCGTCGTCCCGCGCAGAGGC |
| Internal bar code: | GGCAGGTCGATTCTTGGAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 260 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGGCTGCACAATGATAA |
| Suggested primer 2: | AAAGGGTATGCACATTTCGC |