Insertion junction: LMJ.RY0402.062851_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.062851
Chromosome:chromosome_6
Location:7125812
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g297200 FAP108 Flagellar Associated Protein sense 5'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ATTATTCGCAACTCAAGGGTCATTCGCGAC
Internal bar code:ATGTACTGGCTCGCTCACCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.6519
LEAP-Seq n confirming:2863
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:TTATCTACTGCGCGATGTGC
Suggested primer 2:AGTGCGTGAGATTGTTGTGC