Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.062890 |
Chromosome: | chromosome_16 |
Location: | 337966 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g694000 | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATAGCAAACATGTGCGCGACTTCTCAATGC |
Internal bar code: | CGGAAGAGTCTAGTCCAGGATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 886 |
LEAP-Seq percent confirming: | 95.3846 |
LEAP-Seq n confirming: | 124 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCACGTAGAATGTCCGTTT |
Suggested primer 2: | GTCTCCCACTGCGAGTAAGG |