Insertion junction: LMJ.RY0402.062929_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.062929
Chromosome:chromosome 17
Location:1138026
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g704300 FAP47 Flagellar Associated Protein 47; (1 of 1) PTHR23053//PTHR23053:SF2 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // PRIMARY CILIARY DYSKINESIA PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTGCTGGCTGCGGGGTAGAGCTGTTCA
Internal bar code:CGTCCACCAAGTGTGGTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:489
LEAP-Seq percent confirming:99.4851
LEAP-Seq n confirming:10627
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AACAACATAACGGAGCCTGC
Suggested primer 2:TCGATACAGGGTGGTGAACA