Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.062929 |
Chromosome: | chromosome_17 |
Location: | 1138026 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g704300 | FAP47 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGGTTGCTGGCTGCGGGGTAGAGCTGTTCA |
Internal bar code: | CGTCCACCAAGTGTGGTGGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 489 |
LEAP-Seq percent confirming: | 99.4851 |
LEAP-Seq n confirming: | 10627 |
LEAP-Seq n nonconfirming: | 55 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACAACATAACGGAGCCTGC |
Suggested primer 2: | TCGATACAGGGTGGTGAACA |