| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.062930 |
| Chromosome: | chromosome 9 |
| Location: | 4093812 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393580 | (1 of 2) PF03097 - BRO1-like domain (BRO1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCTTGCTGAATGCAGCGCTATAGTCG |
| Internal bar code: | CTACACCCGCTAGCGCGCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 93.8171 |
| LEAP-Seq n confirming: | 3232 |
| LEAP-Seq n nonconfirming: | 213 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGTGGTTGAGACAGTCC |
| Suggested primer 2: | CGAGGAGAAACGCTTGTAGG |