| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.062954 |
| Chromosome: | chromosome 1 |
| Location: | 3251911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020350 | SDH3 | Succinate dehydrogenase subunit b560; (1 of 1) K00236 - succinate dehydrogenase (ubiquinone) cytochrome b560 subunit (SDHC, SDH3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGGTTTTGCGCTCCACCTCCCTACACC |
| Internal bar code: | CGTGTCCAACTGACAGATGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 99.8563 |
| LEAP-Seq n confirming: | 9731 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGAAAGCTCAAAGCCAAG |
| Suggested primer 2: | ATCATCTACCACTGGCTGGG |