| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.062999 |
| Chromosome: | chromosome 5 |
| Location: | 827027 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248000 | CGL29,LESV1 | Like early starvation 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCCTTAGCCATCTCCATTCCAAGGTCC |
| Internal bar code: | AGCGGTGTTTGGACTTAGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 99.3243 |
| LEAP-Seq n confirming: | 147 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGATACAGGACGTGTGC |
| Suggested primer 2: | AAACAAGTTTTGGGTCGTGC |