| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063003 |
| Chromosome: | chromosome 7 |
| Location: | 4496836 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343433 | OGD4 | (1 of 3) 2.3.1.61 - Dihydrolipoyllysine-residue succinyltransferase / Succinyl-CoA:dihydrolipoate S-succinyltransferase; Dihydrolipoamide succinyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCGAGGCACGGGCGGCTTGCATACAG |
| Internal bar code: | CGCTCACCGGGAATCGAGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1693 |
| LEAP-Seq percent confirming: | 88.1188 |
| LEAP-Seq n confirming: | 178 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAGCTACATGCCCTTTA |
| Suggested primer 2: | GAAAGGAGCAGACGACGTTC |