| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063008 |
| Chromosome: | chromosome 6 |
| Location: | 3291798 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCGGTTGAGTTTGCGAACTTGAGGTTG |
| Internal bar code: | ACCAGAAAGTGGGTACGGATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 99.6019 |
| LEAP-Seq n confirming: | 2752 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTATCCATCTTCTGCCG |
| Suggested primer 2: | GATCATGCTCACCAGGAGGT |