| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063050 |
| Chromosome: | chromosome 14 |
| Location: | 3207847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629150 | CYG4 | Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGCAGCAGCACCGGAGGTATTGACGCC |
| Internal bar code: | CAATTTACGGGGGCTTCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 82.399 |
| LEAP-Seq n confirming: | 632 |
| LEAP-Seq n nonconfirming: | 135 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACCTTGTGCTCCTGCT |
| Suggested primer 2: | CGGTGTTTACTGCTGGGTCT |