Insertion junction: LMJ.RY0402.063067_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063067
Chromosome:chromosome 17
Location:4873802
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g734250 (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCCCGCCTCTCACGCGCGCGCAGGGCTC
Internal bar code:ACGAGGTTCAGAAAAAGTTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:925
LEAP-Seq percent confirming:76.2229
LEAP-Seq n confirming:1792
LEAP-Seq n nonconfirming:559
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACATATTTGGTGAGCCCTGC
Suggested primer 2:TAGCACGACAGGATGCTCAC