| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063070 |
| Chromosome: | chromosome 10 |
| Location: | 878229 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423950 | (1 of 1) PTHR12952//PTHR12952:SF0 - SYS1 // PROTEIN SYS1 HOMOLOG-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTCAGGCACTGCCTGCGTCTTAAAAGC |
| Internal bar code: | CAGGGAAACCTGTAGGGACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 99.5639 |
| LEAP-Seq n confirming: | 7077 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCATGATGATGGGAGAT |
| Suggested primer 2: | GCATCGCAGATACGTTGAGA |