| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063084 |
| Chromosome: | chromosome 5 |
| Location: | 227933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233800 | TSG1 | Glycyl-tRNA synthetase; (1 of 1) K01880 - glycyl-tRNA synthetase (GARS, glyS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCTCCCGATGCAGCACTGTCGCCTGCT |
| Internal bar code: | CAGTTAGCGCGATCGACGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 99.5671 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTTCTTACCAGTCCAAA |
| Suggested primer 2: | TATCCGTCTTCGACCCAGTC |