| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063107 |
| Chromosome: | chromosome 12 |
| Location: | 6097788 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535800 | (1 of 1) PF13191//PF13424 - AAA ATPase domain (AAA_16) // Tetratricopeptide repeat (TPR_12) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGCATTGCAAGAGACAGGCTATGCCCT |
| Internal bar code: | ATTGAAGTAGCGGTTTCGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1164 |
| LEAP-Seq percent confirming: | 90.9445 |
| LEAP-Seq n confirming: | 934 |
| LEAP-Seq n nonconfirming: | 93 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAATGGTTGCGTAGTCGGC |
| Suggested primer 2: | AGGCAATGGTCGTACGGTAG |