| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063120 |
| Chromosome: | chromosome 2 |
| Location: | 1674506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTTGGCAAGGGGGGCGGTGCGAGCTGGG |
| Internal bar code: | TCGCTTTAAAGGGGGGGATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 97.6764 |
| LEAP-Seq n confirming: | 4540 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTTTTGTGCGTTTCTGC |
| Suggested primer 2: | TAGACTGCTTCCGTCCGAGT |