| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063128 |
| Chromosome: | chromosome 6 |
| Location: | 2996472 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273500 | DIV61 | (1 of 1) K14641 - apyrase (APY1_2); Apyrase ortholog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCTGCTCATCCGCGCTCATCTCGGTT |
| Internal bar code: | AGATTCCCACGATTATCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1244 |
| LEAP-Seq percent confirming: | 99.7128 |
| LEAP-Seq n confirming: | 9720 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCTACGCCCTCTTGCATT |
| Suggested primer 2: | CAGTCTGGCTTTAAGCTGGG |