| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063137 |
| Chromosome: | chromosome 1 |
| Location: | 5954927 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042352 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCACATCCTCAATTGCACCCTAAATTC |
| Internal bar code: | AGATGGTCCCGGGTGTTGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 99.8316 |
| LEAP-Seq n confirming: | 5336 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGTGGGGGATAAGGAG |
| Suggested primer 2: | GGCAACGAGAACAAGCTTTC |