Insertion junction: LMJ.RY0402.063148_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.063148
Chromosome:chromosome 2
Location:2340391
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g090750 (1 of 1) PF08574 - Protein of unknown function (DUF1762) (DUF1762) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCAACGCGCCGTCGAACCCCTCCAACC
Internal bar code:GATAGTCTAGGTATTGTCCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1108
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:270
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGTCAACAGAGGGGTG
Suggested primer 2:GACTACAGCGAGCACAGCAG