Insertion junction: LMJ.RY0402.063150_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063150
Chromosome:chromosome 3
Location:3940910
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g171600 XPP1,XPP Exopolyphosphatase; (1 of 2) K03787 - 5'-nucleotidase (surE) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCGTGACGGGCGGCCTGGGGCGGCGCC
Internal bar code:TCCGACGTGCGCCAGCATGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:95.9626
LEAP-Seq n confirming:15592
LEAP-Seq n nonconfirming:656
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CTACGGATTGCATGATGGTG
Suggested primer 2:CGTAGGAGTCGCGGTAGAAC