Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.063153 |
Chromosome: | chromosome 8 |
Location: | 1409793 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g364200 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTAGCGTTACCCAGCGATGTCACCAACC |
Internal bar code: | AACGTCATTGATTGGAGTGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 544 |
LEAP-Seq percent confirming: | 99.2703 |
LEAP-Seq n confirming: | 5306 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATTGGACTGAACCTGTGCC |
Suggested primer 2: | CTTCGCTAAACAAAGCCCAG |