Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.063167 |
Chromosome: | chromosome_3 |
Location: | 5255954 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g183200 | CPC1 | Protein associated with central pair microtubule complex | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTTTTGCCGCCTGCAAGCAGCGCCGCCGC |
Internal bar code: | GCTCACGTCGGCATCCTACGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 152 |
LEAP-Seq percent confirming: | 84.0909 |
LEAP-Seq n confirming: | 814 |
LEAP-Seq n nonconfirming: | 154 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTCCAGCGTCTTCCACTC |
Suggested primer 2: | CTGGATCTGGACAGTGAGCA |