| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063173 |
| Chromosome: | chromosome 16 |
| Location: | 6079709 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676050 | ARP3 | (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCCGCCCCTCTCCAGCACGACACCACA |
| Internal bar code: | ATGATCCGGATTGCTGTTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 72.6316 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGCTCGTTCTAGATTGG |
| Suggested primer 2: | GCCGTAACTGTGAGCGTGTA |