| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063179 |
| Chromosome: | chromosome 9 |
| Location: | 4444580 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395621 | DIV122 | (1 of 1) PTHR10933:SF9 - LD07294P | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCATTGCATGGGTGCAAAATTCATGCT |
| Internal bar code: | GCGATGTGGTGGACGACTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 99.4949 |
| LEAP-Seq n confirming: | 197 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGGCTGTAAACATGG |
| Suggested primer 2: | GTCACTAGGGCGCAGAAGTC |