| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063199 |
| Chromosome: | chromosome 16 |
| Location: | 6101817 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675850 | (1 of 1) K11729 - acyl-CoA dehydrogenase family member 10 (ACAD10) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGAAACACACCCTCTGACGCCCCCACCT |
| Internal bar code: | GATACAGCCGTTACTGACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 429 |
| LEAP-Seq percent confirming: | 99.6461 |
| LEAP-Seq n confirming: | 8166 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGTACAGAGAAGTGGGGG |
| Suggested primer 2: | AATGGGCAAGTTTGATGAGG |