| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063230 |
| Chromosome: | chromosome 10 |
| Location: | 17007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g417500 | CYN20E,CYN20-5,CYN9 | (1 of 1) PF00160//PF04969 - Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD (Pro_isomerase) // CS domain (CS); Cyclophilin 20-5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACCGTGAAGTTGTATGCCCTCAGGCAA |
| Internal bar code: | GGATTGTGTTAGGTGCCTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 99.6467 |
| LEAP-Seq n confirming: | 3667 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTCCCCAACCTCCTCT |
| Suggested primer 2: | AGAACCAGCCAGCACTCCTA |