| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063238 |
| Chromosome: | chromosome 12 |
| Location: | 2566469 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505400 | (1 of 1) 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase / Platelet-activating factor acetylhydrolase; Related to platelet-activating factor acetylhydrolase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAATTGGTTGGTTTGGAGGCTGGGCTGG |
| Internal bar code: | GTGAGACCTTCGGCAGGAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 98.7908 |
| LEAP-Seq n confirming: | 6454 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAAGGTTCAATCACGCCC |
| Suggested primer 2: | GCATTAAGGAGGAGACTGCG |