| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063247 |
| Chromosome: | chromosome 12 |
| Location: | 9542410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545602 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACGGCCGCCGATGTCCAGGCCGCTGGT |
| Internal bar code: | CGGATAACTGACACACAGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 70.7758 |
| LEAP-Seq n confirming: | 2308 |
| LEAP-Seq n nonconfirming: | 953 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGCTGGTAGGCAAGAC |
| Suggested primer 2: | ATAAAAGGTGCTCCCCGACT |