Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.063286 |
Chromosome: | chromosome_5 |
Location: | 3171646 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre05.g239500 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCACATCGTGCGCCTGGCGGCGGGCAGCAA |
Internal bar code: | CTGGTGGTTGGCCCAGGCAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 888 |
LEAP-Seq percent confirming: | 99.6119 |
LEAP-Seq n confirming: | 2310 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACTCTTGCTTATAGGGC |
Suggested primer 2: | AGCAAACCAAGAGGCTCGTA |