| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063286 |
| Chromosome: | chromosome 5 |
| Location: | 3171646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239500 | FAP38 | (1 of 1) IPR000104//IPR009068 - Antifreeze protein, type I // S15/NS1, RNA-binding; Flagellar Associated Protein 38 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACATCGTGCGCCTGGCGGCGGGCAGCAA |
| Internal bar code: | CTGGTGGTTGGCCCAGGCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 99.6119 |
| LEAP-Seq n confirming: | 2310 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACTCTTGCTTATAGGGC |
| Suggested primer 2: | AGCAAACCAAGAGGCTCGTA |