| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063291 |
| Chromosome: | chromosome 3 |
| Location: | 4191983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173950 | (1 of 3) PF08495 - FIST N domain (FIST) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTGGCAAACTCGCCCGAGTTCAGCGC |
| Internal bar code: | CCGACGACTTTATGAATCGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 773 |
| LEAP-Seq percent confirming: | 98.0271 |
| LEAP-Seq n confirming: | 9490 |
| LEAP-Seq n nonconfirming: | 191 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGGTGTTTTAAGATGAT |
| Suggested primer 2: | CAACACTCTGTGCTCCCAAA |