| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063301 |
| Chromosome: | chromosome 14 |
| Location: | 1240071 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616400 | (1 of 8) PF00011 - Hsp20/alpha crystallin family (HSP20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCGTATTGCAGCCATCTGAAAGCGGC |
| Internal bar code: | ACGGGTAGTTAAGTGTGCCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 99.7475 |
| LEAP-Seq n confirming: | 790 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATGAGGGTGCAGAGGAG |
| Suggested primer 2: | TGCATAATCTGCTCACTGGC |