| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063324 |
| Chromosome: | chromosome 12 |
| Location: | 4469695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521600 | EEP7 | (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGACGACGTGGATGCATGGAAAGGGT |
| Internal bar code: | CGTTATAATTGCTTGCCAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 189 |
| LEAP-Seq percent confirming: | 6.79183 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 1688 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGTACTATGACGGCGGG |
| Suggested primer 2: | CCATACACACGCATACCTGC |