Insertion junction: LMJ.RY0402.063324_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.063324
Chromosome:chromosome 12
Location:4469791
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g521600 EEP7 (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos); Endonuclease/exonuclease/phosphatase family protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAGTATAATCAAGGTTCCGTCAACCCA
Internal bar code:TACAGCGGGTCAACTCGATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:455
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGTACTATGACGGCGGG
Suggested primer 2:CCATACACACGCATACCTGC