Insertion junction: LMJ.RY0402.063334_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.063334
Chromosome:chromosome 3
Location:7971940
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCTAGGAAGGAACCCATCAGGCTATCACA
Internal bar code:TGGTCCTTGGTACCAACACCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:67
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTGCAGTCCTCCAACTC
Suggested primer 2:TAGCTGACACAGACCGCATC