Insertion junction: LMJ.RY0402.063334_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.063334
Chromosome:chromosome 3
Location:7971944
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g201950 (1 of 1) IPR003882//IPR008752//IPR024079 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin // Metallopeptidase, catalytic domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCATCCCCATGCATAGCATGCCGCAGCG
Internal bar code:GGAACCAGCGTTATGGGTTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:944
LEAP-Seq percent confirming:99.0767
LEAP-Seq n confirming:5151
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTGCAGTCCTCCAACTC
Suggested primer 2:TAGCTGACACAGACCGCATC