| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063338 |
| Chromosome: | chromosome 14 |
| Location: | 4052964 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633650 | PRP6 | (1 of 1) PTHR11246:SF1 - PRE-MRNA-PROCESSING FACTOR 6; Splicing factor, component of the U4/U6-U5 snRNP complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGTGGGATGGAGGGCAATGCATGCAGA |
| Internal bar code: | CAGATGTTTCCGCTCTCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 53 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCCCCTTCCTGTCCTAC |
| Suggested primer 2: | GTGGGAGGGCAGATATGAGA |