| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063344 |
| Chromosome: | chromosome 2 |
| Location: | 3890544 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096100 | (1 of 3) PTHR30545 - PROBABLE DNA-BINDING TRANSCRIPTIONAL REGULATOR SFSA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAATGTTGGCAGTGCGGCGGACAAGTGA |
| Internal bar code: | CTGTTTGGCAAAGCGGAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 98.9332 |
| LEAP-Seq n confirming: | 2133 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACGCCTTCACTTCTT |
| Suggested primer 2: | TACAAGGCAGCATCTACCCC |