| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.063357 |
| Chromosome: | chromosome 2 |
| Location: | 5737018 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110300 | DNJ25 | (1 of 62) PF00226 - DnaJ domain (DnaJ); DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGTTCTCCGCGCCACCGTGGCGGTCCG |
| Internal bar code: | CAACCTTGTGCCCGGTCCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.6023 |
| LEAP-Seq n confirming: | 6011 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTAAGCGGCCTTGATAG |
| Suggested primer 2: | TTTCTGCGACTAGCAAGGGT |