| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063362 |
| Chromosome: | chromosome 3 |
| Location: | 5804645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g189000 | RAD54A,SRH8 | SNF2-related DNA/RNA helicase; (1 of 1) PTHR10799//PTHR10799:SF607 - SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY-RELATED // SNF2-RELATED DOMAIN-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGACAGGCAAGAGCGGGAGGTAACGTA |
| Internal bar code: | GAAAGGCCGGGGCAAATACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 98.8636 |
| LEAP-Seq n confirming: | 2523 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGAAAGGACAAGGAGAG |
| Suggested primer 2: | CAACTCACGTGTTTTCCCCT |