| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063368 |
| Chromosome: | chromosome_6 |
| Location: | 5327703 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g283250 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCCAGGGGGGGGGGTGCGCGGAGTTGTCA |
| Internal bar code: | TTCTGCGAAGAGGATAATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 70.4163 |
| LEAP-Seq n confirming: | 1590 |
| LEAP-Seq n nonconfirming: | 668 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATCTGGTCACAACACAC |
| Suggested primer 2: | CGAAAACAGAAGAAGTCCGC |