| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.063410 |
| Chromosome: | chromosome 12 |
| Location: | 3892911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515850 | PCN1 | (1 of 1) K04802 - proliferating cell nuclear antigen (PCNA); Proliferating cell nuclear antigen homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCAAGGGTGCCAACACCCTCAGCGCTT |
| Internal bar code: | CACAGTTGAGAGTAGGGCTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.8309 |
| LEAP-Seq n confirming: | 2952 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCAGTTGGCGTACGAGG |
| Suggested primer 2: | ACAACCCGAACAAAGAGTGG |