| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063421 |
| Chromosome: | chromosome 12 |
| Location: | 8316822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549750 | FAP4,CK1,CSK1 | Flagellar Associated Protein 4; (1 of 2) K02218 - casein kinase 1 (CSNK1, CK1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGACAAACTCTGACCTCTCCCGCAGGC |
| Internal bar code: | TTATTTTGCTCGTAGCTGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 15.0543 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 1800 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGACTTCTAAGCACGC |
| Suggested primer 2: | CCCGTCCTAGTGGACGTTTA |