| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.063426 |
| Chromosome: | chromosome 17 |
| Location: | 5377712 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCCACACACACGTACGGCGGGGGTACA |
| Internal bar code: | ACTGACAGTCAGATCACAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 972 |
| LEAP-Seq percent confirming: | 80.9594 |
| LEAP-Seq n confirming: | 9333 |
| LEAP-Seq n nonconfirming: | 2195 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCGTGTGCACTGTATG |
| Suggested primer 2: | AAGTATGGCGTGCGGTAATC |