| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063436 |
| Chromosome: | chromosome 12 |
| Location: | 7468254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556200 | (1 of 1) PF15011 - Casein Kinase 2 substrate (CK2S) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGTTGATGTCAGGAGTGTGTGTGTGG |
| Internal bar code: | GTGACCCAAGGAAAGGCGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.7441 |
| LEAP-Seq n confirming: | 8187 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACTTTTGTCCAGGACCG |
| Suggested primer 2: | ATCCGTGCGTTGTTATGTGA |