Insertion junction: LMJ.RY0402.063439_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.063439
Chromosome:chromosome 1
Location:3953199
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g025750 (1 of 1) PF01551 - Peptidase family M23 (Peptidase_M23) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAATGTTGGCTGCGCTGGCTCAACGGAC
Internal bar code:CGCTCGGTTGTCACGACACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.3249
LEAP-Seq n confirming:3384
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCAGTTATCACGTGTGGG
Suggested primer 2:ACGACAGTAACTTGGTGGGG