| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.063479 |
| Chromosome: | chromosome 2 |
| Location: | 5169017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105450 | CGL141 | (1 of 1) PTHR36749:SF1 - F7O18.3 PROTEIN; Conserved in the Green Lineage | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCCGGGCCTGTTCAGCCGGCCCCAGCGG |
| Internal bar code: | AGGACGGGTGCTCGGGGGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 488 |
| LEAP-Seq percent confirming: | 99.4542 |
| LEAP-Seq n confirming: | 10750 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCAACCCAAAGGGATCT |
| Suggested primer 2: | ACCCGGACAAGTTTGTGAAG |